Synaptic Vesicles Caught Kissing Again
نویسنده
چکیده
In this issue of Neuron, Harata et al. use a novel quenching technique to provide compelling evidence that kiss-and-run is the dominant mode of vesicle fusion at hippocampal synapses and that the prevalence of kiss-and-run can be modulated by stimulus frequency. The increased incidence of kiss-and-run at lower frequencies may ensure that vesicles are available for use during periods of high demand.
منابع مشابه
Imaging single synaptic vesicles undergoing repeated fusion events: kissing, running, and kissing again.
At synapses of the mammalian central nervous system, release of neurotransmitter occurs at rates transiently as high as 100 Hz, putting extreme demands on nerve terminals with only tens of functional vesicles at their disposal. Thus, the presynaptic vesicle cycle is particularly critical to maintain neurotransmission. To understand vesicle cycling at the most fundamental level, we studied singl...
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Building on recent findings that synaptotagmin (Syt) participates in synaptic vesicle endocytosis, Poskanzer et al., in this issue of Neuron, show distinct mechanisms by which Syt functions in this process. Most significantly, they show (1) that calcium binding to Syt determines the rate but not fidelity of vesicle recycling and (2) that mutations in a different Syt domain affect the shape but ...
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We describe the design and operation of a machine that freezes biological tissues by contact with a cold metal block, which incorporates a timing circuit that stimulates frog neuromuscular junctions in the last few milliseconds before thay are frozen. We show freeze-fracture replicas of nerve terminals frozen during transmitter discharge, which display synpatic vesicles caught in the act of exo...
متن کاملKissing or Fused Since Some Time
I am particularly interested in the data published by Brown et al. (2010) in their paper entitled " Vesicular release of gluta-mate utilizes the proton gradient between the vesicle and the synaptic cleft ". The paper presents a decrease in transmitter output when the extracellular pH is acidified. The authors interpret their observation in terms of interference with the pH gradient from the ves...
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Synaptic strength depends on the amount of neurotransmitter stored in synaptic vesicles. The vesicular transmitter content has recently been shown to be directly dependent on the expression levels of vesicular neurotransmitter transporters indicating that the transport capacity of synaptic vesicles is a critical determinant for synaptic efficacy.Using synaptic vesicles prepared fromwhole brain ...
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ورودعنوان ژورنال:
- Neuron
دوره 49 شماره
صفحات -
تاریخ انتشار 2006